Proper experimental design requires randomization/balancing of molecular ecology experiments

Proper experimental design requires randomization/balancing of molecular ecology experiments

Properly designed (randomized and/or balanced) experiments are standard in ecological research. Molecular methods are increasingly used in ecology, but studies generally do not report the detailed design of sample processing in the laboratory. This may strongly influence the interpretability of results if the laboratory procedures do not account for the confounding effects of unexpected laborat...

Experimental Investigation of Energy Consumption and Performance of Reverse Osmosis Desalination using Design of Experiments Method

To control the quality of reverse osmosis (RO) product water and reduce operational costs and environmental impacts by increasing the system’s energy efficiency, it is necessary to identify the influence of process parameters on energy consumption and permeate water quality. This paper introduces a case study focused on the application of Design of Experiments (DOE) method in an industrial-scal...

Functional Genomics Requires Ecology

Since Mendel’s time, most genes have been identified by the effects mutations (including knockouts) have on the morphology, physiology, or behavior of individuals. Thus, almost by definition, there could be no ‘‘mutant genes without phenotypes.’’ In the molecular era, however, it has become possible to identify genes from DNA sequences. This alternative path to gene discovery has already led to...

Experimental design of DNA microarray experiments.

Part 2: Experimental design of perturbation experiments

The objectives of in situ experiments studying ocean acidifi cation vary widely, with most recent studies centering on the effects of changing carbonate chemistry on the biology and ecology of organisms and ecosystems. The advantages of in situ observations are that they offer a completely independent approach to laboratory experiments, one that is based on looking directly at how organisms and...